In contrast, when Ai65(RCFL-tdT)-neo mice were first bred with CCK(cholecystokinin)-ires-Cre mice (Stock No.
tdTomato-labeled cells in layer 2/3 show vertically oriented bipolar morphology with irregular spiking (IS) patterns. 010774)) in order to study the fate of progenitor cells at the VIP/CR intersection. 021875) (after removal of the loxP-flanked STOP codon by breeding with CR(calretinin)-ires-cre mice (Stock No. These mice were bred to Ai65(RCFL-tdT)-neo mice (Stock No. As such, when VIP-ires-Flp mice are bred with mice containing frt-flanked sequence(s), Flp-mediated recombination will result in deletion of the flanked sequence(s) in the Vip-expressing cells of the offspring. FLP is detected in the neocortex, hippocampus, olfactory bulb, suprachiasmatic nuclei, and other discrete midbrain and brainstem regions. They represent up to 15% of all cortical interneurons. VIP expressing cells are GABAergic neurons derived from the caudal-ganglionic eminence (CGE). VIP-ires-Flp knock-in mice express Flp Recombinase from the endogenous vasoactive intestinal polypeptide ( Vip) promoter/enhancer elements.
